Целью настоящей работы являлось изу

Целью настоящей работы являлось изучение особенностей введения в культуру in vitro видов Paeonia hybrida Pall. и P.anomala L., редких и исчезающих видов флоры Казахстана. Paeonia hybrida Pall. (Paeoniaceae Rudolphi) – эндемик юго-востока Западной Сибири и гор Восточного Казахстана. Встречается в горах Алтая, Тарбагатае, Сауре, на северном склоне Джунгарского Алатау, а также в прилегающей части Восточного мелкосопочника. P.anomala L. (Paeoniaceae Rudolphi) – редкий и исчезающий вид, занесен в Красную книгу животных и растений Республики Казахстан. Произрастает преимущественно в Казахстане, Средней Азии, Алтае, в лесной зоне Сибири от Урала до Лены и Байкала, очень редко - на севере европейской части России. Численность и ареалы данных видов сокращаются из-за сбора растений на букеты, выкапывания корней для использования в целях лечения. В связи с этим задачей исследования являлось введение в культуру in vitro P.hybridа и P.anomala, для дальнейшего микроклонального размножения и использования полученного материала для сохранения и восстановления численности популяций данных видов.
Одним из важных моментов на этапе введения в культуру является выбор концентрации и экспозиции используемого стерилизующего агента. Были разработаны эффективные приемы стерилизации семян Paeonia hybrida и P.anomala и почек возобновления Р. hybrida.
Разработаны приемы введения в культуру и культивирования in vitro семян и изолированных зародышей P. hybridа и P.anomala.
Показана возможность размножения в культуре P. hybridа из эксплантов почек возобновления. На этапе микроразмножения для представителей P.hybrida Pall оптимальными являются питательные среды, дополненные 1 мг/л БАП, 1 мг/л ИУК и 40 г/л сахарозы. Применение среды Мурасиге-Скуга с половинной концентрацией солей, комплексом регуляторов роста (БАП, ИУК, ГА3) и 40 г/л сахарозы, положительно на этапе введения почек P.hybrida в культуру и на этапе микроразмножения.
При использовании в качестве эксплантов для введения в культуру семян P. hybridа и P.anomala эффективным оказалось изолирование зародыша. На этапе введения в культуру зародышей P.anomala эффективна среда Мурасиге-Скуга с добавлением ИУК 1 мг/л, БАП 1 мг/л, кинетина 1 мг/л, гиббереловой кислоты 0,1 мг/л.
Разработанные приемы получения растений-регенерантов пиона гибридного флоры Восточного Казахстана методами биотехнологии будут использованы для сохранения генофонда в коллекциях in vitro; полученные растения путем клонального микроразмножения будут адаптированы к открытому грунту, исследования будут продолжаться с дальнейшей интродукцией и реинтродукций в природные популяции.
Ключевые слова: культура тканей и органов растений, клональное микроразмножение, сохранение биоразнообразия, эндемичные виды.

Результаты (английский) 1:

The aim of this work was to study the features of the introduction of in vitro culture species Paeonia hybrida Pall. and p. l. anomala, rare and endangered species of flora of Kazakhstan. Paeonia hybrida Pall. (Paeoniaceae Rudolphi) is endemic to the South-East of Western Siberia and the mountains of the Eastern Kazakhstan. Found in the mountains of Altai, Saur, Tarbagatai, on the northern slope of the Jungar Alatau, as well as adjacent parts of East hills. P. l. anomala (Paeoniaceae Rudolphi) is a rare and endangered species, is listed in the Red data book of animals and plants of the Republic of Kazakhstan. It grows mainly in Kazakhstan, Central Asia, Altai, Siberia in the forest zone from the Urals to the Lena and Lake Baikal, very rarely, in the North of European part of Russia. The number and data types of habitats are shrinking due to collect plants on bouquets, digging up roots for use in treatment. In this regard, the objective of the study was the introduction of in vitro culture p. hybrida and p. anomala, Micropropagation and use the resulting material for the preservation and restoration of populations of the species.One of the highlights at the stage of introduction of culture is the choice of the concentration and exposure used sterilizing agent. Effective sterilization techniques were developed seeds of Paeonia hybrida and p. anomala and kidneys resume r. hybrida.Developed techniques for introduction in culture and in vitro cultivation of seeds and isolated embryos p. hybrida and p. anomala. The possibility of breeding a culture of p. hybrida kidney explants of renewal. On stage micro propagation for representatives of p. hybrida Pall optimal are nourishing Wednesday, supplemented with 1 mg/l BENZYLAMINOPURINE, 1 mg/l IAA and 40 g/l sucrose. Application of Murasige-Skog Wednesday with half the concentration of salts, plant growth regulators (BAP, IAA, GA3) and 40 g/l sucrose, positively on the stage of kidney p. hybrida in culture and micro propagation phase.When used as explants for introducing the culture of p. hybrida seeds and p. anomala effective proved isolating embryo. At the stage of introduction to culture embryos p. anomala effective Wednesday Murasige-Skog with addition of IPC 1 mg/l, 1 mg/l BENZYLAMINOPURINE, kinetin 1 mg/l gibberelovoj acid 0.1 mg/l.Developed receptions of getting the plants-regenerants Peony hybrid flora of Eastern Kazakhstan biotechnology techniques are used to preserve the gene pool in collections in vitro; obtained by micro propagation of clonal plants are adapted to the open ground, the research will continue with further introduction and reintrodukcij in natural populations.Keywords: culture tissues and organs of plants, Micropropagation, biodiversity conservation, endemic species.

Результаты (английский) 2:

The aim of this work was to study the characteristics of the introduction of an in vitro culture of species Paeonia hybrida Pall. and P.anomala L., rare and endangered species of flora in Kazakhstan. Paeonia hybrida Pall. (Paeoniaceae Rudolphi) - endemic to the southeast of Western Siberia and Eastern Kazakhstan mountains. It is found in the mountains of Altai, Tarbagatay, Saur, on the northern slope of Jungar Alatau, as well as in the adjacent part of East Upland. P.anomala L. (Paeoniaceae Rudolphi) - a rare and endangered species, listed as endangered animals and plants of the Republic of Kazakhstan. It grows mainly in Kazakhstan, Central Asia, the Altai, in the forest zone of Siberia from the Urals to the Lena and Lake Baikal, very rarely - in the north of European Russia. Number and habitats of these species are reduced because of the collection of plants for bouquets, digging roots for use in treatment. In this regard, the task of the study was the introduction to the culture in vitro and P.hybrida P.anomala, for further micropropagation and the use of the material obtained for the preservation and restoration of the population of these species populations.
One of the highlights at the stage of introduction in culture is the choice of concentration and used sterilant exposure. Effective methods of sterilization of seeds and Paeonia hybrida P.anomala and kidneys resuming R. hybrida were developed.
Developed methods of introduction in culture and in vitro cultivation of seeds and isolated embryos P. hybrida and P.anomala.
The possibility of breeding in P. hybrida culture of explants resuming kidneys. At the stage of micropropagation for representatives P.hybrida Pall are optimal culture media supplemented with 1 mg / l BAP, 1 mg / l IAA and 40 g / l sucrose. Application of Murashige and Skoog medium with half strength salts, growth regulators complex (BAP, IAA, n3) and 40 g / l of sucrose step positively introducing P.hybrida kidney culture and micropropagation phase.
When used as explants for administration culture and seeds P. hybrida P.anomala effective was the isolation of the embryo. In step introduction in culture embryos P.anomala effective Murashige-Skoog medium supplemented with IAA, 1 mg / l BAP 1 mg / l Kinetin 1 mg / l, gibberelovoy acid 0.1 mg / l.
The developed techniques obtain regenerated plants peony hybrid flora of East Kazakhstan biotechnology methods will be used to preserve the gene pool in the collections of in vitro; plants obtained by clonal micropropagation will be adapted to the open ground, the study will continue with the further introduction and reintroduction in natural populations.
Key words: culture of tissues and organs of plants, clonal micropropagation, conservation of biodiversity, endemic species.

Результаты (английский) 3:

the purpose of the present work was to study the characteristics of the culture in vitro of Paeonia hybrida pall. and P.anomala l., rare and endangered species of flora in kazakhstan. Paeonia hybrida pall. (Paeoniaceae Rudolphi endemism is south east of west siberia and the mountains east of kazakhstan. found in the mountains of altai, тарбагатае, сауре, on the north side of the джунгарского alatau, as well as in the adjacent part of the eastern hills. P.anomala l. (Paeoniaceae Rudolphi) is a rare and disappearing species listed in the red book of animals and plants of the republic of kazakhstan. grows mainly in kazakhstan, central asia, siberia, altai, in the forest zone of the urals to the lena and baikal, very rarely, in the north of the european part of russia. the number of species and habitats are reduced because of the collection of plants, flowers, выкапывания roots for use for the treatment. therefore, the research has been the introduction of culture in vitro P.hybridа and P.anomala, for further микроклонального reproduction and use of the material for the preservation and restoration of populations of species.one of the highlights of the implementation phase of the culture is the choice of concentration and exposure of the стерилизующего agent. have developed effective techniques for sterilization of seeds Paeonia hybrida and P.anomala and kidneys of r. hybrida.developed the techniques of in vitro culture and cultivation of seeds and isolated embryos p. hybridа and P.anomala.the reproduction of culture in p. hybridа of kidney эксплантов resumption. at the stage of микроразмножения for representatives of P.hybrida pall optimal are nutritional environment, supplemented with 1 mg / l bap, 1 mg / l иук and 40 g / l сахарозы. the application of environment мурасиге - скуга with half the concentration of salts, the combination of growth regulators (bap, иук, га3) and 40 g / l сахарозы, positive phase of kidney P.hybrida in culture and on the stage микроразмножения.with the use of a эксплантов for the culture of seeds of p. hybridа and P.anomala effective was isolating the embryo. at the stage of introduction into the culture of embryos P.anomala effective environment мурасиге - скуга appendix иук 1 mg / l bap 1 mg / l, кинетина 1 mg / l, гиббереловой acid 0.1 mg / l.the techniques of регенерантов peony plants flora of east kazakhstan hybrid methods of biotechnology will be used to preserve the gene pool in the collections of plants through in vitro; the клонального микроразмножения will be adapted to the open ground, the research will continue with the further introduction and реинтродукций in natural populations.key words: culture of tissues and organs of plants, клональное микроразмножение, conservation of biodiversity of endemic species.